Serial Dilution Calculator
Plan serial dilution steps from stock concentration and dilution factor for lab protocols.
Serial Dilution Calculator
The Serial Dilution Calculator helps laboratory professionals and researchers plan multi-step dilution protocols. Serial dilutions are commonly used in microbiology, biochemistry, and pharmacology to create a range of concentrations from a single stock solution.
In a serial dilution, each step dilutes the previous solution by a constant factor (dilution factor). The concentration at any step n is:
$$C_n = \frac{C_0}{DF^n}$$
where C0 is the initial concentration, DF is the dilution factor per step, and n is the step number. This calculator helps you determine the volumes needed for each dilution step.
For related calculations, try our Cell Dilution Calculator and Solution Dilution Calculator.
Frequently Asked Questions
How do you calculate serial dilution?
In a serial dilution, each step multiplies the dilution factor. If you have a 1:10 serial dilution, the first step is 1:10, the second is 1:100, the third is 1:1000, and so on. The final concentration = initial concentration / (dilution factor)^(number of steps).
What is the purpose of serial dilution?
Serial dilutions are used to create a wide range of concentrations from a small volume of stock solution. They are essential for determining bacterial colony counts (CFU/mL), creating standard curves for assays, finding minimum inhibitory concentrations (MIC) in antimicrobial testing, and reducing sample concentration to a measurable range.
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